Establishment of Lysogeny in Bacteriophage 186
نویسندگان
چکیده
منابع مشابه
Establishment of lysogeny in bacteriophage 186. DNA binding and transcriptional activation by the CII protein.
The CII protein of bacteriophage 186 is a transcriptional activator of the helix-turn helix family required for establishment of the lysogenic state. DNA binding by 186 CII is unusual in that the invertedly repeated half sites are separated by 20 base pairs, or two turns of the DNA helix, rather than the one turn usually associated with this class of proteins. Here, we investigate quantitativel...
متن کاملGenes for the establishment and maintenance of lysogeny by the temperate coliphage 186.
To identify the genes in coliphage 186 that are required for lysogeny, we isolated clear-plaque mutants. Complementation studies and DNA sequencing identified two genes, the cI gene for the immunity maintenance repressor and the cII gene, which is required only for the establishment of lysogeny. One mutant carried a change in the LexA-binding site controlling expression of the antirepression pr...
متن کاملRevisiting Bistability in the Lysis/Lysogeny Circuit of Bacteriophage Lambda
The lysis/lysogeny switch of bacteriophage lambda serves as a paradigm for binary cell fate decision, long-term maintenance of cellular state and stimulus-triggered switching between states. In the literature, the system is often referred to as "bistable." However, it remains unclear whether this term provides an accurate description or is instead a misnomer. Here we address this question direc...
متن کاملLysogeny and bacteriophage host range within the Burkholderia cepacia complex.
The Burkholderia cepacia complex comprises a group of nine closely related species that have emerged as life-threatening pulmonary pathogens in immunocompromised patients, particularly individuals with cystic fibrosis or chronic granulomatous disease. Attempts to explain the genomic plasticity, adaptability and virulence of the complex have paid little attention to bacteriophages, particularly ...
متن کاملOrigin and direction of replication of bacteriophage 186 DNA.
Intracellular bacteriophage 186 DNA replicates as a single-branched circle during the first round of replication. The free end of the branch is located at a unique position with respect to phage 186 DNA base sequence, and this point should, therefore, correspond to the origin of DNA replication. The position of the growing point has been mapped at various degrees of replication, and found to mo...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2000
ISSN: 0021-9258
DOI: 10.1074/jbc.m004574200